Custom Peptide Modifications Guidance

Best Biochem offers over 400 kinds of N- and C-terminal and internal peptide modifications to help you produce the custom peptides that you need to propel your research forward. This table separates modifications based on type to help you quickly determine which modification will best suit your needs. 500+ different modifications have been successfully synthesized with peptides in Best Biochem.

  1. D form, and non-naturalamino acid

  2. Fluorescent dyesfor protein binding or localization studies as well as emitting and quenching dyes for Förster resonance energy transfer

  3. Carrier proteinsor MAPs(Multiple antigenic peptides) to prepare peptide antigen

  4. Cyclization including disulfide bridge, head to tail cyclic to make peptide stabilization

  5. Spacersor linker like Acp, PEG

  6. Stable isotope labeling with heavy amino acids modification

Peptide ModificationName Abbr.
N-terminal modificationBiotinBiotin-
N-terminal modificationCholesterol(Chol)-
N-terminal modification Vitamin E succinale(vitamin E succinate)-
N-terminal modification 6-Hydr azinonicotinic acid(Hynic)-
N-terminal modification Ferrocenecaroxylic acid(FCA)-
N-terminal modification 4-Chloro-7-nitro-1,2,3-benzoxadiazoleNBD
N-terminal modification CarboxybenzylZ/Cbz
N-terminal modification BenzylBzl/Bn
N-terminal modification BenzoylBz
N-terminal modification Fluorescein isothiocyanate5-FITC-
N-terminal modification Rhodamine BRhodamine B-
N-terminal modification 5-Carboxytetramethylrhodamine5-TAMRA-
N-terminal modification 5-Carboxyfluorescein5-FAM-
N-terminal modification Fluorescein disodium saltFluorescein
N-terminal modification Fluorescein-5-maleimide(Mal-FAM)-
N-terminal modification 7-Methoxycoumarin-4-acetic acid(Mca)-
N-terminal modification 4-(4-DiMethylaMinophenyl)(Dabcyl)-
N-terminal modification Dansyl(Dansyl)-
N-terminal modification Formyl(Formyl)-
N-terminal modification AcetylAc-
N-terminal modification Succinyl(Suc)
N-terminal modification n-Pentanoic acid(C5)-
N-terminal modification 1,5-Pentanedioic/Glutaric acid(GAA)-
N-terminal modification Octanoic acid(C8)-
N-terminal modification Capric acid(C10)-
N-terminal modification Lauric acid(C12)-
N-terminal modification Tridecanoic acid(C13)-
N-terminal modification Myristic acid(Myr)-
N-terminal modification Palmitic acid(Pal)-
N-terminal modification Stearic acid(C18)-
N-terminal modification n-heneicosanoic acid(C21)-
N-terminal modification Crotonic acid/Trans-crotonic acid
N-terminal modification M-bromobenzoic acid(FB)-
N-terminal modification 1-Adamantane Carboxylic Acid(ACA)-
N-terminal modification indole-3-acetic acid
N-terminal modification 1-Naphthaleneacetic acid
N-terminal modification 2-Naphthaleneacetic acid
N-terminal modification 1-naphthyloxyacetic acid
N-terminal modification 2-naphthyloxyacetic acid
N-terminal modification Lactic acidLactic acid
N-terminal modification Oleic acidOleic acid
N-terminal modification Linoleic acidLinoleic acid
N-terminal modification trans-Cinnamic acidtrans-Cinnamic acid
N-terminal modification Acrylic acidAcrylic acid
N-terminal modification 2-methyl-acrylic acid(MAA)
N-terminal modification cis-4,7,10,13,16,19-Docosahexaenoic acid(DHA)
C-terminal modification C-terminal amidation-NH2
C-terminal modification Coumarin 120/4-Methyl-7-aminocoumarin(AMC)
C-terminal modification Coumarin 151/7-Amion-4-(triflioromethyl) coumarin(AFC)
C-terminal modification 4-Nitroaniline(PNA)
C-terminal modification β-Naphthylamin(β-Naphthylamin)
C-terminal modification EthylamineNHET
C-terminal modification MethylamineNHMe
C-terminal modification methyl ester-OMe
C-terminal modification cysteamine/mercaptoethylamine-NHCH2CH2SH
C-terminal modificationacyl hydrazine-NH-NH2
Special amino acids β-alanine(β-A)
Special amino acids L-Pyroglutamin acid(Glp)/(Pyr)
Special amino acids Citrulline(Cit)
Special amino acids L-Norvaline(Nva)
Special amino acids Norleucine(Nle)
Special amino acids L-Hydroxyproline(Hyp)
Special amino acids OrnithineO
Special amino acids D-Ornithine(D-Orn)
Special amino acids 6-Aminocaproic acid(Ahx)/(Acp)
Special amino acids 2-amino isobutyric acid(Aib)
Special amino acids 2-amniobutyrin acid(Abu)
Special amino acids γ-aminobutyric acidGABA/γ-Abu
Special amino acids [DAP(Dnp)]/Dpa
Special amino acids 2,3-Diaminopropionic acid(Dap)
Special amino acids L-2,4-Diaminobutyric acid(Dab)
Special amino acids D-2,4-Diaminobutyric acid(D-Dab)
Special amino acids L-3,3-diphenylalanine(Dip)
Special amino acids D-3,3-diphenylalanine(D-Dip)
Special amino acids L-4,4'-biphenylalanine(Bip)
Special amino acids D-4,4'-biphenylalanine(D-Bip)
Special amino acids 3-Benzothienylalanine(Bal)
Special amino acids (2S)-2-amino-3-(4-benzoylphenyl) propanoic acid(Bpa)
Special amino acids (2R)-2-amino-3-(4-benzoylphenyl) propanoic acid(D-Bpa)
Special amino acids L-1-Naphthylalanine(1-Nal)
Special amino acids L-2-Naphthylalanine(2-Nal)
Special amino acids D-2-Naphthylalanine[D-(2-Nal)]
Special amino acids 3-Cyclohexyl-L-alanine methyl ester(Cha)
Special amino acids 3-Cyclohexyl-D-alanine methyl ester(D-Cha)
Special amino acids 1-aminocyclohexanecarboxylic acid(1-aminocyclohexanecarboxylic acid)
Special amino acids 1,2-Piperidinedicarboxylic acid(1,2-Piperidinedicarboxylic acid)
Special amino acids L-Phenylglycine(Phg)
Special amino acids D-alpha-Cyclohexylglycine(D-Chg)
Special amino acids L-alpha-Cyclohexylglycine(Chg)
Special amino acids Anthranilic acid(2-Abz)
Special amino acids 3-Aminobenzoic acid(3-Abz)
Special amino acids 4-Aminobenzoic acid(4-Abz)
Special amino acids L-methionine sulfoxide[M(O)]
Special amino acids L-Methionine sulfone[M(O2)]
Special amino acids 3-chloro-L-phenylalanine[F(3-Cl)]
Special amino acids 4-chloro-L-phenylalanine[F(4-Cl)]
Special amino acids L-4-Fluorophenylalanine[F(4-F)]
Special amino acids L-4-Methylphenylalanine[F(4-Me)]
Special amino acids 3-Nitro-L-tyrosine[Y(3-NO2)]
Special amino acids 3-chlorine-L-tyrosine[Y(3-Cl)]
Special amino acids 2-amino-3-(3,4-dihydroxyphenyl)propanoic acid(DOPA)
Special amino acids 2,6-Dimethyl-L-tyrosine(DMT)
Special amino acids L-Thiazolidine-4-carboxylic acid(Thc)/(Thz)
Special amino acids S-Acetamidomethyl-L-Cysteine[C(Acm)]
Special amino acids L-Penicillamine(L-Pen)
Special amino acids D-Penicillamine(D-Pen)
Special amino acids 1-azidocyclopropane-1-carboxylic acid(AC3C)
Special amino acids N'-(Carboxymethyl) diethylenetriamine-N,N,N'',N''-tetraacetic acid tetra-tert-butyl ester(DTPA)
Special amino acids 3-azido-L-ala(AZA)
Special amino acids L-1,2,3,4-Tetrahydroisoquinoline-3-carboxylic acid(Tic)
Stable isotope labeled peptides Arg(13C6,15N4)
Stable isotope labeled peptides Ile(13C6,15N)
Stable isotope labeled peptides Lys(13C6,15N2)
Stable isotope labeled peptides Val(13C5,15N)
Azide modification (2S)-2-Amino-4-azido-butanoic acid(Aha)
Azide modification [K(N3)]
Azide modification 2-Azidoacetic acid(2-Azido)
Azide modification 3-Azidopropionic acid(3-Azido)
Azide modification 5-Azidopentanoic acid(5-Azido)
Azide modification 6-Azido-hexanoic acid(6-Azido)
PEG modification NH2-PEG2-CH2COOH(AEEA)
PEG modification NH2-PEG2-CH2CH2COOH(PEG2)
PEG modification NH2-PEG3-CH2CH2COOH(PEG3)
PEG modification NH2-PEG4-CH2CH2COOH(PEG4)
PEG modification NH2-PEG5-CH2COOH(PEG5)
PEG modification NH2-PEG6-CH2CH2COOH(PEG6)
PEG modification NH2-PEG8-CH2CH2COOH(PEG8)
PEG modification NH2-PEG12-CH2CH2COOH(PEG12)
PEG modification NH2-PEG24-CH2CH2COOH(PEG24)
PEG modification N3-PEG4-CH2CH2COOH(N3-PEG4)
PEG modification N3-PEG12-CH2CH2COOH(N3-PEG12)
PEG modification N3-PEG24-CH2CH2COOH(N3-PEG24)
PEG modification CH3O-PEG2000-CH2CH2COOH(mPEG2000)
PEG modification CH3O-PEG5000-CH2CH2COOH(mPEG5000)
PEG modification NH2-PEG2000-CH2CH2COOH(PEG2000)
PEG modification DSPE-PEG2000-COOH(DSPE-PEG2000)
PEG modification DSPE-PEG2000-Mal(DSPE-PEG2000-Mal)
Alkynyl modification Propiolic acid(3-Alkynyl)
Alkynyl modification 5-Hexynoic acid(5-Alkynyl)
Alkynyl modification 4-Pentynoic acid(4-Alkynyl)
Alkynyl modification L-Propargylglycine(L-Pra)
Alkynyl modification D-Propargylglycine(D-Pra)
Sulfur modification 3-Mercaptopropionic acid(Mpa)
Sulfur modification 4-Thiobutyric acid
Sulfur modification 6-Mercaptohexanoic acid(MHA)
Macrocyclic Carboxylic Acid 1,4,7-triazacyclononane-N,N',N''-triacetic acid(NOTA)
Macrocyclic Carboxylic Acid1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-trtraaceticacid(DOTA)
Mal modification 3-Maleimidopropionic acid(3-Mal)/(MIPA)
Mal modification 4-Maleimidobutyric acid(4-Mal)
Mal modification 6-Maleimidocaproic acid(6-Mal)
Mal modification 4-Maleimidobenzoic acid