| Product Name | Caspase 3 (Apopain) Substrate 1f, fluorogenic |
| Purity | HPLC>95% |
| Description | AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm. Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1 |
| Storage | -20°C |
| References | Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63. |
| Molecular Weight | 729.5 |
| Sequence (One-Letter Code) | Ac-DEVD-AFC |
| Sequence (Three-Letter Code) | Ac - Asp - Glu - Val - Asp - AFC |
Caspase 3 (Apopain) Substrate 1f, fluorogenicAdmin2021-01-05T08:38:03+00:00
