| Product Name | BNP-45, mouse |
| Purity | HPLC>95% |
| Description | BNP-45 represents the 45 amino acids at the C-terminus and the mouse BNP-45 has all the amino acid residues thought essential for NP bioactivity, although sequence identity when studied with other BNP hormones (rat, 64%; dog, 53%; pig, 50%; and human, 44%) was clearly less than the identity among ANF hormones. Further, sequence identity between rat and mouse BNP prohormones is found to be more conserved in the N-terminal portion of the prohormone than in the C-terminal BNP-45 (88% versus 64%). A protein kinase C phosphorylation site is found to be present in the putative mature mouse BNP-45 hormone at threonine 81. Comparing the amino acid sequence surrounding the proteolytic processing site for BNP-32 in human, pig, and dog BNP with the corresponding site for BNP-45 in the rat and mouse sequence we find that all of the secreted BNP hormones have an N-terminal serine preceded by an arginine in the prohormone sequence, and the mouse and rat sequences are highly conserved at the putative scissile bond (LKRVLR-SQ). Further comparative sequence analysis indicates that an arginine being present at position -4 relative to the scissile (R-S) bond in all mammalian BNP precursors. Thus, processing of BNP prohormones to both BNP-45 in rodents and BNP-32 in higher mammals appears to require a protease with a conserved recognition sequence (RXXR-S). Also, the conserved sequences in the BNP prohormones matches the consensus cleavage site for human furin, a calcium-dependent serine endoprotease expressed in mouse heart, and possibly having a role in processing BNP precursors. |
| Storage | -20°C |
| References | Steinhelper, ME. Circ. Res. 72: 984-992. (1993). |
| Molecular Weight | 4919.6 |
| Sequence (One-Letter Code) | SQGSTLRVQQRPQNSKVTHISSCFGHKIDRIGSVSRLGCNALKLL (Disulfide bridge:23-39) |
| Sequence (Three-Letter Code) | H - Ser - Gln - Gly - Ser - Thr - Leu - Arg - Val - Gln - Gln - Arg - Pro - Gln - Asn - Ser - Lys - Val - Thr - His - Ile - Ser - Ser - Cys - Phe - Gly - His - Lys - Ile - Asp - Arg - Ile - Gly - Ser - Val - Ser - Arg - Leu - Gly - Cys - Asn - Ala - Leu - Lys - Leu - Leu - OH (Disulfide bridge:23 - 39) |
BNP-45, mouseAdmin2021-01-04T11:58:10+00:00
